3D biological samples are submitted to a permanent flow of solvent which ensures that the solution is transferred to their core. A constant supply of reactant is pumped through the chamber ensuring that no stagnation occurs and that the integrity of the samples is preserved
CONSTANT FLOW OF REACTANT
QUARTZ FLOW CELLS
ABILITY TO MULTI-TASK
Four individual experiments can be carried out simultaneously with different probes and temperatures. For example, it is possible to test in one session, a probe with four different hybridization temperatures.
RECYCLING PROCESS
COMPACT SIZE
Constant recycling of the solvent results in ARN and antibody probes that are ten times weaker than normal which ensures that background signaling is minimized and helps to reduce significantly the time that is needed to wash samples. The probes are collected at the end of the session and can be re-used for future experiments.
Product Benefits
Flogentec is an automated device that can determine with a high degree of accuracy, the expression of genes or proteins in biological samples. Whereas mRNAs are specifically targeted using the Wholemount In Situ Hybridization (WISH) principle, proteins are detected with specific antibodies and secondary antibodies.
Biological specimens are placed in the basket which is made of a plastic ring, at the bottom of which is a sieve. Many of them are stacked in a column made of quartz (the so called Flow Cell). Solution flow within the Flow cells, the quartz incubation chambers, is driven from the bottom to the top, using a system made up of reservoirs, tubing, a pump, and selector valves managed by computer hardware. This dynamic incubation process in combination with a precise temperature control system ensures the efficient transfer of probes and antibodies to the samples' core and optimizes the washing of samples, reducing dilution levels of solvents and increasing the overall efficiency of the process.
The final result is a highly efficient, user-friendly, automatic system which can radically improve both the speed and the quality of Wholemount mapping research projects.
The schematic below demonstrates the key product benefits of this device
Reduced lead times & higher quality results :
The benefits of the Flogentec System
The Flogentec device works entirely on low voltage (24 Volts). Its compact size makes it easy to use.
The incubation chamber or "flow cell" is made out of quartz which helps ensure highly precise levels of thermoregulation. The chamber can hold between 10 to 20 samples. However, dependent on their size, between 40 and 300 samples can be analyzed in one experiment in 24 hours.
TOTAL AUTONOMY
The user settings for each experiment are imported into the Flogentec device via a USB port. The machine is then able to function in its own right without the need to be connected to a PC. Once the experiment has finished, a summary of the session can be exported back to the PC via the USB port for further analysis.
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